Rapid evolution and host immunity drive the rise and fall of carbapenem resistance during an acute Pseudomonas aeruginosa infection

  06 May 2021

It is well established that antibiotic treatment selects for resistance, but the dynamics of this process during infections are poorly understood. Here we map the responses of Pseudomonas aeruginosa to treatment in high definition during a lung infection of a single ICU patient. Host immunity and antibiotic therapy with meropenem suppressed P. aeruginosa, but a second wave of infection emerged due to the growth of oprD and wbpM meropenem resistant mutants that evolved in situ. Selection then led to a loss of resistance by decreasing the prevalence of low fitness oprD mutants, increasing the frequency of high fitness mutants lacking the MexAB-OprM efflux pump, and decreasing the copy number of a multidrug resistance plasmid. Ultimately, host immunity suppressed wbpM mutants with high meropenem resistance and fitness. Our study highlights how natural selection and host immunity interact to drive both the rapid rise, and fall, of resistance during infection.

Further reading: Nature Communications
Author(s): Rachel Wheatley, Julio Diaz Caballero, Natalia Kapel, Fien H. R. de Winter, Pramod Jangir, Angus Quinn, Ester del Barrio-Tofiño, Carla López-Causapé, Jessica Hedge, Gabriel Torrens, Thomas Van der Schalk, Basil Britto Xavier, Felipe Fernández-Cuenca, Angel Arenzana, Claudia Recanatini, Leen Timbermont, Frangiscos Sifakis, Alexey Ruzin, Omar Ali, Christine Lammens, Herman Goossens, Jan Kluytmans, Samir Kumar-Singh, Antonio Oliver, Surbhi Malhotra-Kumar & Craig MacLean
Healthy Patients   Smart Innovations  


Unrestricted financial support by:


Antimicrobial Resistance Fighter Coalition

Bangalore Bioinnovation Centre



Every two weeks in your inbox

Because there should be one newsletter that brings together all One Health news related to antimicrobial resistance: AMR NEWS!


What is going on with AMR?
Stay tuned with remarkable global AMR news and developments!

Keep me informed